Coding
TnsC

Part:BBa_K3776018

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)



TnsC

This TnsC protein from the E.coli Tn7 is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see BBa_K3776022). TnsC is a ATPase which regulates target site selection.(Klompe et al., 2019)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 312
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 231
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None